Biocatalysis using S9 ω-transaminase from Escherichia coli
نویسنده
چکیده
منابع مشابه
Combination of ester biosynthesis and ω-oxidation for production of mono-ethyl dicarboxylic acids and di-ethyl esters in a whole-cell biocatalytic setup with Escherichia coli
BACKGROUND Medium chain length (C6-C12) α,ω-dicarboxylic acids (DCAs) and corresponding esters are important building blocks for the polymer industry. For DCAs of 12 carbon atoms and longer, a sustainable process based on monooxygenase catalyzed ω-oxidation of fatty-acids has been realized. For medium-chain DCAs with a shorter chain length however, such a process has not been developed yet, sin...
متن کاملA novel mediatorless microbial fuel cell based on direct biocatalysis of Escherichia coli.
A mediatorless microbial fuel cell based on the direct biocatalysis of Escherichia coli shows significantly enhanced performance by using bacteria electrochemically-evolved in fuel cell environments through a natural selection process and a carbon/PTFE composite anode with an optimized PTFE content.
متن کاملSurface expression of ω-transaminase in Escherichia coli.
Chiral amines are important for the chemical and pharmaceutical industries, and there is rapidly growing interest to use transaminases for their synthesis. Since the cost of the enzyme is an important factor for process economy, the use of whole-cell biocatalysts is attractive, since expensive purification and immobilization steps can be avoided. Display of the protein on the cell surface provi...
متن کاملIdentification by affinity chromatography of the rat liver ribosomal proteins that bind to Escherichia coli 5 S ribosomal ribonucleic acid.
The eukaryotic and prokaryotic ribosomal proteins that bind to Escherichia coli 5 S rRNA were identified by affinity chromatography. The E. coli ribosomal proteins that associated with the nucleic acid were L5, L18, and L25 confirming earlier findings using the same and different procedures. The rat liver ribosomal proteins that associated with E. coli 5 S rRNA were L6, L7, L19, L35a, and S9; s...
متن کاملBiocatalytic Synthesis of Amino - Alcohols Using a de novo Designed Transketolase - P - Alanine : Pyruvate Transaminase Pathway in Escherichia coli
Biocatalysis continues to emerge as a powerful technique for the efficient synthesis of optically pure pharmaceuticals that are difficult to access via conventional chemistry. The power of a biocatalytic stage can be enhanced if two or more reactions can be achieved by a single whole cell biocatalyst containing a pathway designed de novo to facilitate a required synthetic sequence. This will al...
متن کامل